A trial of tablet substitutes for the Gregersen test.

Method 2.-As regards Method 2, however, once the time taken for the cells to sickle has been discovered by the wet preparation, one tube can be set up for the equivalent time, and, using the quantities stated, upwards of 24 films can be prepared, all evenly spread. It follows that if permanent preparations are desired to show the stages of sickling formation this second method is most suitable. The method of choice for regular, reliable, and most presentable preparations is as follows: (1) Set up wet preparation and at the same time set up one tube as described in method 2 above. (2) When the cells in the wet preparation have sickled, add formalin to the tube. (3) After -fixation is complete prepare films and stain as described above.